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Category
Proteins
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Antibody Subtype
Recombinant Proteins
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Area of research
Proteases, Inhibitors, & Enzymes
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Reactivity
E.coli
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Residues
1-491 amino acids: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 90% pure
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Concentration
1 mg/ml (lot specific)
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Form Buffer
50 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, and 10% glycerol.
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Storage
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE
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Bioactivity
Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 DEG C.
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Assay Information
1. Prepare a 3ml reaction cocktail into a suitable container: The final concentrations are 50mM gylcylglycine, 2mM D-glucose 6-phosphate, 0.67mM beta-NADP, 10mM MgCl2. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 4ug of recombinant G6PD into reaction cocktail and mix immediately. 4. Record the increase at A340nm for 5 minutes.
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Additional Information
G6PD protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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