EGF protein

Category

Proteins
Antibody Subtype

Recombinant Proteins
Area of research

Cytokines & Growth Factors
Reactivity

Human
Residues

MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
Type of protein

Recombinant
Expression System

Escherichia coli
Grade Purity

Grater than 95% pure
Concentration

1 mg/ml (lot specific)
Form Buffer

Provided as a liquid in PBS, pH 7.4.
Storage

Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditions

Blue Ice
Tested for

SDS-PAGE
Bioactivity

The ED50 for this effect is < 0.89 ng/ml . Measured in a cell proliferation assay using mouse Balb3T3 cell.
Assay Information

Cell line: Balb-3T3 (Mus musculus (mouse)) Maintenance Condition: RPMI 1640 containing 10% FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation time: 24 hr (after sample treatment) Concentration range: 1 pg/ml - 1 ug/ml Detection method: BrdU assay
Additional Information

EGF protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
URL
More info

Gene target

EGF protein
Gene symbol

EGF
Short name

EGF protein
Alternative name

Purified recombinant Human EGF protein
Alternative to gene target

epidermal growth factor, HOMG4 and URG, EGF and IDBG-34100 and ENSG00000138798 and 1950, transmembrane receptor protein tyrosine kinase activator activity, Extracellular, Egf and IDBG-193432 and ENSMUSG00000028017 and 13645