Donkey anti Human IgG H + L HRP
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Catalog number
43R-ID037hrp
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Price
Please ask
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Size
500 µl
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Donkey anti-human IgG (H + L) (HRP) was raised in donkey using human IgG (H&L) ..
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Raised in
Donkey
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Specificity
Human
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
HRP
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Method of Purification
Donkey anti Human IgG (H + L) (HRP) was purified by affinity chromatography.
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Form Buffer
Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA. Reconstitute with 0.5 ml of distilled Water.
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Storage
Store at 4 deg C.
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Shipping conditions
Blue Ice
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Tested for
ELISA; ICC; IHC; WB
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Usage Recommendations
ELISA: 1:5,000-1:100,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, WB: 1:10,000-1:200,000
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Latin name
Equus africanus asinus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Gene symbol
CALD1, FUT1, L3MBTL2
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Short name
anti IgG H + L
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Technique
anti human, anti, IgG, antibody to, anti human antibodies, IgGs
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Host
Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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Isotype
IgG, IgG
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Label
HRP
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Species
Human, Donkeys, Humans
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Alternative name
Donkey anti Human IgG (H + L) secondary antibody (HRP)
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Alternative technique
antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
caldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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