Cyclophilin A protein His tag
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Catalog number
80R-1031
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Price
Please ask
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Size
100 µg
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Category
Proteins
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Antibody Subtype
Conjugated Proteins
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Area of research
Cell Cycle & Cell Death
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Reactivity
Human
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Residues
1-165 amino acids: MGSSHHHHHH SSGLVPRGSH MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE
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Conjugate Tag
His tag
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 95% pure
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Concentration
1 mg/ml (lot specific)
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Form Buffer
Provided as a liquid in 20 mM Tris, pH 8.0, containing 20 mM NaCl, 0.5 mM DTT, and 10% glycerol.
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Storage
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE
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Bioactivity
Specific activity is > 200 nmoles/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 1C in Tris-Hcl pH8.0 using chymotrypsin.
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Assay Information
1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Cyclophilin A protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
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Additional Information
Cyclophilin A protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Conjugation
histidine
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