CD86 antibody biotin
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Catalog number61R-CD86aHUBT
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PricePlease ask
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Size100 µg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchImmunology
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Type of ImmunogenCD86 antibody (biotin) was raised in mouse using ARH-77 (B-lymphoblastiod cell line) ..
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Raised inMouse
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SpecificityHuman
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Cross ReactivityShould be determined by the end user
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IsotypesIgG1
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CloneBU63
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Conjugate TagBiotin
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Grade PurityGrater than 95% pure
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Method of PurificationCD86 antibody (biotin) was purified by Protein A chromatography.
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Concentration1.0 mg/ml
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Form Buffer50 mM Na3PO4, pH 7.5, with 100 mM KCL, 150 mM NaCl, 5% Glycerol, 0.2% BSA, and 0.04% NaN3.
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StorageStore at 4 deg C.
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Shipping conditionsBlue Ice
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Tested forELISA; FC; WB
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Usage RecommendationsFC: 5 ug/ml
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Additional InformationThis antibody recognizes the CD86 molecule of 80 kd. It blocks MLR and blocks binding of soluble CD152-muIg fusion protein to CD86.
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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French translationanticorps
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Gene target
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Gene symbolCD86
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Short nameCD86 antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelBiotin
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Alternative nameMouse monoclonal CD86 antibody (biotin)
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Alternative techniqueantibodies
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Alternative to gene targetCD86 molecule, B7-2 and B7.2 and B70 and CD28LG2 and LAB72, CD86 and IDBG-52336 and ENSG00000114013 and 942, coreceptor activity, Cell surfaces, Cd86 and IDBG-158071 and ENSMUSG00000022901 and 12524, CD86 and IDBG-633264 and ENSBTAG00000013118 and 414345
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Gene info
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Identity
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Gene
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Long gene nameCD86 molecule
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Synonyms gene
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Synonyms gene name
- CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- B7 family
- CD molecules
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data