CD69 antibody FITC
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Catalog number61R-1104
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PricePlease ask
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Size50 µg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchImmunology
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Raised inHamster
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SpecificityMouse
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IsotypesIgG
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CloneH1.2F3
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Conjugate TagFITC
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Concentration200 ug/mL
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Form BufferProvided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
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StorageStore at 2-8 deg C. Do not freeze. Protect from light.
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Shipping conditionsBlue Ice
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Tested forFC
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Usage RecommendationsFC: 5 ul (0.5 ug)/test
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Assay InformationA test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test.
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolCD69
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Short nameCD69 antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameArmenian Hamster monoclonal CD69 antibody (FITC)
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Alternative techniqueantibodies, fluorescine
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Alternative to gene targetCD69 molecule, AIM and BL-AC/P26 and CLEC2C and EA1 and GP32/28 and MLR-3, CD69 and IDBG-18077 and ENSG00000110848 and 969, carbohydrate binding, Cell surfaces, Cd69 and IDBG-191849 and ENSMUSG00000030156 and 12515, CD69 and IDBG-639618 and ENSBTAG00000002135 and 281058
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Gene info
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Identity
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Gene
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Long gene nameCD69 molecule
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Synonyms gene name
- CD69 antigen (p60, early T-cell activation antigen)
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-08-06
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Entrez gene record
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Pubmed identfication
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Classification
- C-type lectin domain containing
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data