CD4 T cell receptor antibody biotin
-
Catalog number
61-003005
-
Price
Please ask
-
Size
50 µg
-
-
Category
Primary Antibody
-
Antibody Subtype
Conjugated Monoclonal Antibodies
-
Area of research
Immunology
-
Type of Immunogen
CD4 antibodies were raised in mouse using full length recombinant CD4 (T cell) ..
-
Raised in
Mouse
-
Isotypes
IgG1
-
Clone
45
-
Conjugate Tag
Biotin
-
Grade Purity
Grater than 95% pure
-
Concentration
50 ug/vial
-
Form Buffer
Provided lyophilized as a purified IgG in PBS buffer, with no preservatives added, reconstitute in sterile distilled water.
-
Storage
Store lyophilized product at 2-8 deg C short term, following reconstitution, aliquot and store at -70 deg C or lower. Avoid repeated freeze/thaw cycles
-
Shipping conditions
Blue Ice
-
Tested for
ELISA; WB
-
Usage Recommendations
ELISA: 1:500-1:1000, WB: 1:500-1:1000
-
-
-
Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
Conjugation
Biotinylated
-
Description
CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. For cells, cell lines and tissues in culture till half confluency. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
-
French translation
anticorps
-
Gene target
-
Gene symbol
CD4
-
Short name
CD4 T receptor antibody
-
Technique
Antibody, antibodies against human proteins, antibodies for
-
Label
Biotin
-
Alternative name
Mouse monoclonal CD4 antibody (biotin)
-
Alternative technique
antibodies
-
Alternative to gene target
CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
-
Tissue
cell
-
Gene info
-
Identity
-
Gene
-
Long gene name
CD4 molecule
-
Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
-
GenBank acession
-
Locus
-
Discovery year
1986-01-01
-
Entrez gene record
-
RefSeq identity
-
Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products