CD28 antibody biotin

Category

Primary Antibody
Antibody Subtype

Conjugated Monoclonal Antibodies
Area of research

Immunology
Raised in

Mouse
Specificity

Human
Isotypes

IgG1 kappa
Clone

CD28.2
Conjugate Tag

Biotin
Concentration

500 ug/ml
Form Buffer

Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
Storage

Store at 2-8 deg C. Do not freeze. Protect from light.
Shipping conditions

Blue Ice
Tested for

FC
Usage Recommendations

FC: ≤ 0.25 ug/test
Assay Information

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Gene target

CD28
Gene symbol

CD28
Short name

CD28 antibody
Technique

Antibody, antibodies against human proteins, antibodies for
Label

Biotin
Alternative name

Mouse monoclonal CD28 antibody (biotin)
Alternative technique

antibodies
Alternative to gene target

CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050

Identity

HGNC:1653
Gene

CD28
Long gene name

CD28 molecule
Synonyms gene name
- CD28 antigen (Tp44)
Synonyms name
- T-cell-specific surface glycoprotein
GenBank acession
- J02988
Locus

2q33.2
Discovery year

1988-05-11
Entrez gene record

940
Pubmed identfication
- 1355979
RefSeq identity
- NM_006139
Classification
- CD molecules
- Ig-like cell adhesion molecule family
- V-set domain containing
VEGA ID

OTTHUMG00000132878

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data