Carbonic anhydrase II protein
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Catalog number
30R-1325
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Price
Please ask
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Size
100 µg
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Category
Proteins
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Antibody Subtype
Recombinant Proteins
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Area of research
Proteases, Inhibitors, & Enzymes
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Reactivity
Human
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Residues
1-260 amino acids: MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 95% pure
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Concentration
1 mg/ml (lot specific)
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Form Buffer
Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 1 mM DTT, 50 mM NaCl, and 10% glycerol.
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Storage
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE
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Bioactivity
Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37 deg C.
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Assay Information
1. Prepare assay buffer into a suitable container: The final concentrations are 12.5 mM Tris, 75 mM NaCl, pH 7.5.2. Dilute 4-Nitrophenyl-Acetate(4-NPA) to 2 mM in Assay Buffer. 3. Dilute recombinant CA2 protein with various concentrations (1ug, 2ug, 5ug) in 50 ul assay buffer. 4. Load 50 uL of 2mM 4-NPA into diluted recombinant CA2 solutions, and start the reaction by adding 50 uL of 2 mM Substrate. 5. Read at a wavelength of 405 nm (bottom read) in kinetic mode for 5 minutes.
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Additional Information
Carbonic anhydrase II protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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