Avidin antibody biotin
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Catalog number60C-CF0103
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PricePlease ask
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Size1 ml
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Polyclonal Antibodies
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Area of researchMiscellaneous
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Type of ImmunogenAvidin antibody (biotin) was raised in rabbit using avidin isolated from hen egg white ..
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Raised inRabbit
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SpecificityChicken avidin
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Cross ReactivityShould be determined by the end user
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Conjugate TagBiotin
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Method of PurificationAvidin antibody (biotin) was purified by ion exchange chromatography followed by dialysis.
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Form BufferProvided as a liquid in 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.2, 10 mg/ml BSA (IgG and Protease free), with 0.01% NaN3 as a preservative.
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StorageStore at 4 deg C or aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forELISA; IHC; WB
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Usage RecommendationsELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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French translationanticorps
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Gene target
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Short nameAvidin antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelBiotin
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Alternative nameRabbit polyclonal Avidin antibody (biotin) conjugated
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data