Alkaline Phosphatase antibody E. Coli HRP

Category

Primary Antibody
Antibody Subtype

Conjugated Polyclonal Antibodies
Area of research

Proteases, Inhibitors, & Enzymes
Type of Immunogen

Alkaline phosphatase antibody (HRP) was raised in rabbit using alkaline phosphatase isolated from E.coli ..
Raised in

Rabbit
Specificity

E Coli alkaline phosphotase
Cross Reactivity

Should be determined by the end user
Conjugate Tag

HRP
Method of Purification

Alkaline Phosphatase antibody (E. Coli) (HRP) was purified by ion exchange chromatography followed by dialysis.
Form Buffer

Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. I mMunoglobulin and protease free.
Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Shipping conditions

Blue Ice
Tested for

ELISA; WB
Usage Recommendations

ELISA: 1:4,000-1:20,000, WB: 1:500-1:2,000
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Conjugation

HRP Conjugated
French translation

anticorps

Gene target

Alkaline Phosphatase
Gene symbol

ALPP, ALPI, ALPG
Short name

Alkaline Phosphatase antibody
Technique

Antibody, antibodies against human proteins, antibodies for
Host

e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. fitzgerald supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
Label

Alkaline Phosphatase
Species

E. coli, E. coli
Alternative name

Rabbit polyclonal Alkaline Phosphatase antibody (E. Coli) (HRP) conjugated
Alternative technique

antibodies, escherichia

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data