AKR1C1 protein His tag

• Catalog number
  80R-1267
• Price
  Please ask
• Size
  100 µg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Proteases, Inhibitors, & Enzymes
• Reactivity
  Human
• Residues
  1-323 amino acids: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
• Conjugate Tag
  His tag
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 90% pure
• Concentration
  0.5 mg/ml (lot specific)
• Form Buffer
  Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 1 mM DTT and 20% glycerol.
• Storage
  Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  SDS-PAGE
• Bioactivity
  Specific activity: approximately 0.15 - 0.2 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 DEG C.
• Assay Information
  1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1C1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25 DEG C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.
• Additional Information
  AKR1C1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
• URL
• More info
• Conjugation
  histidine

• Gene target
  AKR1C1   protein   His   tag  
• Gene symbol
  AKR1C1
• Short name
  AKR1C1 protein His tag
• Technique
  Tags
• Label
  His
• Alternative name
  Purified recombinant Human AKR1C1 protein

Gene info

• Identity
  HGNC:384
• Gene
  AKR1C1
• Long gene name
  aldo-keto reductase family 1 member C1
• Synonyms gene
  • DDH1
• Synonyms gene name
  • aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase)
• Synonyms
  • DDH
  • MBAB
  • DD1
  • HAKRC
• Synonyms name
  • dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase
• GenBank acession
  • D26124
• Locus
  10p15.1
• Discovery year
  1994-09-14
• Entrez gene record
  1645
• Pubmed identfication
  • 8011662
• RefSeq identity
  • NM_001353
• Classification
  • Aldo-keto reductases
• VEGA ID
  OTTHUMG00000017580

Similar products