Actin antibody biotin

• Catalog number
  61R-A108aBT
• Price
  Please ask
• Size
  20 µg

• Category
  Primary Antibody
• Antibody Subtype
  Conjugated Monoclonal Antibodies
• Area of research
  Cell Biology
• Type of Immunogen
  Actin antibody (biotin) was raised in mouse using N-Terminal decapeptide of alpha smooth muscle isoform of actin, acetylated at the N-terminus, ..
• Raised in
  Mouse
• Cross Reactivity
  Baboon, chicken, cow, human, mouse, rabbit, rat
• Isotypes
  IgG2a kappa
• Clone
  1A4
• Conjugate Tag
  Biotin
• Method of Purification
  Actin antibody (biotin) was purified by Protein G chromatography.
• Concentration
  40 ug/ml
• Form Buffer
  Antibody purified from ascites fluid by protein G chromotography and Provided in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% NaN3.
• Storage
  Store at 4 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  IF; IHC-F; IHC-P
• Usage Recommendations
  IHC-F: 1:800, IHC-P: 1:800
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  Actin  
• Gene symbol
  ACTB, ACTG1P10, ACTR3
• Short name
  Actin antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Label
  Biotin
• Alternative name
  Mouse monoclonal Actin antibody (biotin)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:132
• Gene
  ACTB
• Long gene name
  actin beta
• Synonyms name
  • β-actin
• GenBank acession
  • M28424
• Locus
  7p22.1
• Discovery year
  1986-01-01
• Entrez gene record
  60
• Pubmed identfication
  • 1505215
• RefSeq identity
  • NM_001101
• Classification
  • PBAF complex
  • BAF complex
  • GBAF complex
  • Actins
• VEGA ID
  OTTHUMG00000023268
• Locus Specific Databases
  • LRG_132

Gene info

• Identity
  HGNC:155
• Gene
  ACTG1P10
• Long gene name
  actin gamma 1 pseudogene 10
• Synonyms gene
  • ACTL1
  • ACTGP10
• Synonyms gene name
  • actin-like 1
  • actin, gamma pseudogene 10
• GenBank acession
  • AL139396
• Locus
  Xp11.22
• Discovery year
  1986-01-01
• Entrez gene record
  83
• Pubmed identfication
  • 6592095
• RefSeq identity
  • NG_003039
• VEGA ID
  OTTHUMG00000021601

Gene info

• Identity
  HGNC:170
• Gene
  ACTR3
• Long gene name
  actin related protein 3
• Synonyms gene name
  • ARP3 (actin-related protein 3, yeast) homolog
  • ARP3 actin-related protein 3 homolog (yeast)
  • ARP3 actin related protein 3 homolog
• Synonyms
  • ARP3
• GenBank acession
  • AF006083
• Locus
  2q14.1
• Discovery year
  1999-05-17
• Entrez gene record
  10096
• Pubmed identfication
  • 9230079
• RefSeq identity
  • NM_005721
• Classification
  • Actin related protein 2/3 complex subunits
  • Actin related proteins
• VEGA ID
  OTTHUMG00000153497

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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