anti- NCL antibody

Form

liquid
Purification

Immunogen affinity purified
Purity

≥95% as determined by SDS-PAGE
Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Immunogen

nucleolin
Calculated MW

110 kDa
Uniprot ID

P19338
Specificity

Human, Mouse, Rat
Tested Application

ELISA, WB, IHC
Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Image1

Immunohistochemistry of paraffin-embedded human prostate cancer using FNab05587(NCL antibody) at dilution of 1:100
Image4

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab05587(NCL antibody) at dilution of 1:1000
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Gene target

NCL
Gene symbol

CAPN8, CAPN9, NCL, CAPN5, CAPN3
Short name

anti- NCL antibody
Technique

Antibody, anti, antibody to, antibodies against human proteins, antibodies for
Host

Rabbit
Isotype

IgG
Alternative name

antibody to- NCL (antibody to-)
Alternative technique

antibodies

Identity

HGNC:7667
Gene

NCL
Long gene name

nucleolin
Synonyms
- C23
- Nsr1
Locus

2q37.1
Discovery year

1991-01-15
Entrez gene record

4691
Pubmed identfication
- 2394707
- 3409881
RefSeq identity
- NM_005381
Classification
- Small nucleolar RNA protein coding host genes
- SSU processome
- DNA helicases
- RNA binding motif containing
VEGA ID

OTTHUMG00000153866

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data