anti- CAD antibody

Form

liquid
Purification

Immunogen affinity purified
Purity

≥95% as determined by SDS-PAGE
Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Calculated MW

243 kDa
Uniprot ID

P27708
Specificity

Human
Tested Application

ELISA, WB, IHC
Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Gene target

CAD
Gene symbol

CALD1, CAD, B4GALNT2, ACOD1, CDH12P2, DFFB
Short name

anti- CAD antibody
Technique

Antibody, anti, antibody to, antibodies against human proteins, antibodies for
Host

Rabbit
Isotype

IgG
Alternative name

antibody to- CAD (antibody to-)
Alternative technique

antibodies

Identity

HGNC:1424
Gene

CAD
Long gene name

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms
- GATD4
GenBank acession
- D78586
Locus

2p23.3
Discovery year

2001-06-22
Entrez gene record

790
Pubmed identfication
- 8619816
- 2565865
Classification
- Glutamine amidotransferase class 1 domain containing
VEGA ID

OTTHUMG00000097070

Identity

HGNC:24136
Gene

B4GALNT2
Long gene name

beta-1,4-N-acetyl-galactosaminyltransferase 2
Synonyms gene
- GALGT2
Synonyms gene name
- UDP-GalNAc:Neu5Acalpha2-3Galbeta-R beta1,4-N-acetylgalactosaminyltransferase
Synonyms
- Sda
- Cad
GenBank acession
- AJ517770
Locus

17q21.32
Discovery year

2004-02-20
Entrez gene record

124872
Pubmed identfication
- 8782649
- 12678917
RefSeq identity
- NM_153446
Classification
- Beta 4-glycosyltransferases
VEGA ID

OTTHUMG00000161307

Identity

HGNC:1752
Gene

CDH12P2
Long gene name

cadherin 12 pseudogene 2
Synonyms gene
- CDH12P
Synonyms gene name
- cadherin 12 (N-cadherin 2) pseudogene
- cadherin 12 (N-cadherin 2) pseudogene 2
Synonyms
- C41-CAD
GenBank acession
- AC004999
Locus

5q13.2
Discovery year

1995-11-30
Entrez gene record

1011
Pubmed identfication
- 7731968
- 10369862
VEGA ID

OTTHUMG00000162392

Identity

HGNC:2773
Gene

DFFB
Long gene name

DNA fragmentation factor subunit beta
Synonyms gene name
- DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
Synonyms
- CAD
- CPAN
- DFF-40
- DFF40
Synonyms name
- caspase-activated DNase
Locus

1p36.32
Discovery year

1997-10-27
Entrez gene record

1677
Pubmed identfication
- 9108473
- 9560346
RefSeq identity
- NM_001282669
VEGA ID

OTTHUMG00000003525

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data