ZAP-70 PAb

• Catalog number
  11-430-C100
• Price
  Please ask
• Size
  100ug

• Long name
  Rabbit Polyclonal Antibody against Human ZAP-70, Clone: PAb
• Antigen
  ZAP-70
• Species reactivity
  Human (Homo sapiens)
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Polyclonal Antibody (PAb)
• Clone
  Polyclonal
• Applications
  WB
• Immunogen
  Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag
• Format conjugation
  purified; unconjugated
• Specificity
  The polyclonal antibody recognizes C-terminal part of human ZAP-70 protein tyrosine kinase. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products._x000D_ _x000D_
• Research area
  Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine)
• Concentration
  1 mg/ml
• Storage buffer
  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Shipping and storage
  Rabbit Polyclonal Antibody against Human ZAP-70, Clone: PAb is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified from rabbit serum by affinity chromatography
• Datasheet
• References
  Contact us for the most recent list of references

• Gene target
  ZAP-70   PAb  
• Gene symbol
  ZAP70, IGHV2-70, IGLVI-70, YLPM1, RNU6-70P, SWAP70, HLA-K, UCHL5, ZC3HAV1
• Short name
  Anti-ZAP-70 PAb
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  IgG (various subtypes)
• Alternative name
  Rabbit Polyclonal to ZAP-70

Gene info

• Identity
  HGNC:12858
• Gene
  ZAP70
• Long gene name
  zeta chain of T cell receptor associated protein kinase 70
• Synonyms gene
  • SRK
• Synonyms gene name
  • SRK
• Synonyms
  • ZAP-70
  • STD
• Synonyms name
  • tyrosine-protein kinase ZAP-70
• GenBank acession
  • L05148
• Locus
  2q11.2
• Discovery year
  1995-05-16
• Entrez gene record
  7535
• Pubmed identfication
  • 1423621
• RefSeq identity
  • NM_001079
• Classification
  • SH2 domain containing
  • Syk family tyrosine kinases
• VEGA ID
  OTTHUMG00000153060
• Locus Specific Databases
  • ZAP70base: Mutation registry for autosomal recessive ZAP70 immunodeficiency
  • LRG_126

Gene info

• Identity
  HGNC:5577
• Gene
  IGHV2-70
• Long gene name
  immunoglobulin heavy variable 2-70
• GenBank acession
  • L21969
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28454
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000191972

Gene info

• Identity
  HGNC:5940
• Gene
  IGLVI-70
• Long gene name
  immunoglobulin lambda variable (I)-70 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable (I)-70
  • immunoglobulin lambda variable (I)-70 pseudogene
• Synonyms
  • IGLV(I)-70
• GenBank acession
  • D86993
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28763
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000150834

Gene info

• Identity
  HGNC:17798
• Gene
  YLPM1
• Long gene name
  YLP motif containing 1
• Synonyms gene
  • C14orf170
• Synonyms gene name
  • chromosome 14 open reading frame 170
• Synonyms
  • ZAP
  • PPP1R169
• Synonyms name
  • protein phosphatase 1, regulatory subunit 169
• GenBank acession
  • AK090435
• Locus
  14q24.3
• Discovery year
  2003-11-21
• Entrez gene record
  56252
• Pubmed identfication
  • 7596406
• RefSeq identity
  • NM_019589
• Classification
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000172503

Gene info

• Identity
  HGNC:42560
• Gene
  RNU6-70P
• Long gene name
  RNA, U6 small nuclear 70, pseudogene
• Synonyms gene
  • RNU6-70
• Synonyms gene name
  • RNA, U6 small nuclear 70
• Locus
  13q12.2
• Discovery year
  2011-08-08
• Entrez gene record
  106478988

Gene info

• Identity
  HGNC:17070
• Gene
  SWAP70
• Long gene name
  switching B cell complex subunit SWAP70
• Synonyms gene name
  • SWAP switching B-cell complex 70kDa subunit
  • SWAP switching B-cell complex subunit 70
• Synonyms
  • KIAA0640
  • SWAP-70
• Synonyms name
  • switch-associated protein 70
• GenBank acession
  • AB014540
• Locus
  11p15.4
• Discovery year
  2009-05-20
• Entrez gene record
  23075
• Pubmed identfication
  • 9734811
  • 10681448
• RefSeq identity
  • NM_015055
• Classification
  • EF-hand domain containing
  • Pleckstrin homology domain containing
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000165865

Gene info

• Identity
  HGNC:4969
• Gene
  HLA-K
• Long gene name
  major histocompatibility complex, class I, K (pseudogene)
• Synonyms gene
  • HLAK
  • HLA70
• Synonyms
  • HLA-70
• GenBank acession
  • M96335
• Locus
  6p22.1
• Discovery year
  1993-11-02
• Entrez gene record
  3138
• Pubmed identfication
  • 1517564
• RefSeq identity
  • NG_002398
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031162
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:19678
• Gene
  UCHL5
• Long gene name
  ubiquitin C-terminal hydrolase L5
• Synonyms gene name
  • ubiquitin carboxyl-terminal hydrolase L5
• Synonyms
  • UCH37
  • CGI-70
  • INO80R
• Synonyms name
  • INO80 complex subunit R
• Locus
  1q31.2
• Discovery year
  2002-11-11
• Entrez gene record
  51377
• Pubmed identfication
  • 10810093
  • 16027725
• RefSeq identity
  • NM_015984
• Classification
  • Ubiquitin C-terminal hydrolases
  • INO80 complex
• VEGA ID
  OTTHUMG00000035561

Gene info

• Identity
  HGNC:23721
• Gene
  ZC3HAV1
• Long gene name
  zinc finger CCCH-type containing, antiviral 1
• Synonyms
  • ZAP
  • FLB6421
  • FLJ13288
  • MGC48898
  • ZC3HDC2
  • ZC3H2
  • PARP13
  • ARTD13
• Synonyms name
  • zinc finger antiviral protein
  • CCCH-type zinc finger antiviral protein
• GenBank acession
  • BX571742
• Locus
  7q34
• Discovery year
  2003-12-11
• Entrez gene record
  56829
• Pubmed identfication
  • 12215647
  • 12851707
  • 25635049
• RefSeq identity
  • NM_020119
• Classification
  • Poly(ADP-ribose) polymerases
  • Zinc fingers CCCH-type
• VEGA ID
  OTTHUMG00000157471

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