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Long name
Mouse Monoclonal Antibody against Human TCR beta (Vbeta5.3-related), Clone: MEM-262
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Antigen
TCR beta (Vbeta5.3-related)
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Species reactivity
Other not tested, Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
MEM-262
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Applications
FC, IP, WB, FUNC
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Immunogen
Human thymoma cell line HPB-ALL.
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Format conjugation
low endotoxin, unconjugated
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Specificity
The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This_x000D_ subset is larger than that recognized by other V(beta5.3)-specific antibodies.
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Research area
CD and Related Antigens (Human)
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Concentration
1 mg/ml
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Storage buffer
Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
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Shipping and storage
Mouse Monoclonal Antibody against Human TCR beta (Vbeta5.3-related), Clone: MEM-262 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Pavlistova D, Drbal K, Hilgert I, Horejsi V: A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett. 2003 Aug 5;88(2):105-8. _x000D_
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
