SIT monoclonal antibody

• Catalog number
  11-345-C100
• Price
  Please ask
• Size
  100ug

• Long name
  Mouse Monoclonal Antibody against Human SIT, Clone: SIT-01
• Antigen
  SIT
• Species reactivity
  Human (Homo sapiens)
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  SIT-01
• Applications
  FC, IP, WB
• Immunogen
  Bacterially produced recombinant intracellular fragment of human SIT.
• Format conjugation
  purified; unconjugated
• Specificity
  The antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.
• Research area
  Adaptor Proteins (Human), Adaptor Proteins
• Concentration
  1 mg/ml
• Storage buffer
  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Shipping and storage
  Mouse Monoclonal Antibody against Human SIT, Clone: SIT-01 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  SIT (SHP2-interacting transmembrane adaptor_x000D_ protein) is expressed exclusively in lymphoid_x000D_ organs and acts either as a positive or as a negative_x000D_ regulatory element in T cell activation and in T_x000D_ cell development. Binding to Grb2 plays a pivotal_x000D_ role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors._x000D_
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by protein-A affinity chromatography
• Datasheet
• References
  *Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21. , *Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  SIT   monoclonal  
• Gene symbol
  SIT1, HHAT
• Short name
  Anti-SIT Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG1
• Alternative name
  Mouse Monoclonal to SIT
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:17710
• Gene
  SIT1
• Long gene name
  signaling threshold regulating transmembrane adaptor 1
• Synonyms gene name
  • suppression inducing transmembrane adaptor 1
• Synonyms
  • SIT
• Synonyms name
  • SHP2 interacting transmembrane adaptor
• Locus
  9p13.3
• Discovery year
  2005-04-25
• Entrez gene record
  27240
• Pubmed identfication
  • 11491537
  • 10209036
• RefSeq identity
  • NM_014450
• VEGA ID
  OTTHUMG00000019867

Gene info

• Identity
  HGNC:18270
• Gene
  HHAT
• Long gene name
  hedgehog acyltransferase
• Synonyms
  • FLJ10724
  • MART-2
  • MART2
  • Skn
  • ski
  • rasp
  • sit
  • GUP2
• Synonyms name
  • protein-cysteine N-palmitoyltransferase HHAT
• GenBank acession
  • AK001586
• Locus
  1q32.2
• Discovery year
  2004-09-15
• Entrez gene record
  55733
• Pubmed identfication
  • 11160356
• RefSeq identity
  • NM_018194
• Classification
  • Membrane bound O-acyltransferases
• VEGA ID
  OTTHUMG00000036447

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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