SIT monoclonal antibody
#
-
Catalog number11-345-C100
-
Price:
-
Size100ug
-
-
Long nameMouse Monoclonal Antibody against Human SIT, Clone: SIT-01
-
AntigenSIT
-
Species reactivityHuman (Homo sapiens)
-
Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
-
ClonalityMonoclonal Antibody (Mab)
-
CloneSIT-01
-
ApplicationsFC, IP, WB
-
ImmunogenBacterially produced recombinant intracellular fragment of human SIT.
-
Format conjugationpurified; unconjugated
-
SpecificityThe antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.
-
Research areaAdaptor Proteins (Human), Adaptor Proteins
-
Concentration1 mg/ml
-
Storage bufferPhosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
-
Shipping and storageMouse Monoclonal Antibody against Human SIT, Clone: SIT-01 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
-
BackgroundSIT (SHP2-interacting transmembrane adaptor_x000D_ protein) is expressed exclusively in lymphoid_x000D_ organs and acts either as a positive or as a negative_x000D_ regulatory element in T cell activation and in T_x000D_ cell development. Binding to Grb2 plays a pivotal_x000D_ role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors._x000D_
-
Purity> 95% (by SDS-PAGE)
-
Purification methodPurified by protein-A affinity chromatography
-
References*Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21. , *Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
-
PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translationanticorps
-
Gene target
-
Gene symbolSIT1, HHAT
-
Short nameAnti-SIT Mab
-
TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
-
HostMouse (Mus musculus)
-
IsotypeIgG1
-
Alternative nameMouse Monoclonal to SIT
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namesignaling threshold regulating transmembrane adaptor 1
-
Synonyms gene name
- suppression inducing transmembrane adaptor 1
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year2005-04-25
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namehedgehog acyltransferase
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2004-09-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Membrane bound O-acyltransferases
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data