PRKAR2A monoclonal antibody

• Catalog number
  11-428-C025
• Price
  Please ask
• Size
  25ug

• Long name
  Mouse Monoclonal Antibody against Human PRKAR2A, Clone: Hs-36
• Antigen
  PRKAR2A
• Species reactivity
  Human (Homo sapiens)
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  Hs-36
• Applications
  ICC, WB
• Immunogen
  Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Format conjugation
  purified; unconjugated
• Specificity
  The antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), previously identified under the general name "intra-acrosomal protein". _x000D_
• Research area
  Fertility & Reproduction (Human), Fertility & Reproduction
• Concentration
  1 mg/ml
• Storage buffer
  Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
• Shipping and storage
  Mouse Monoclonal Antibody against Human PRKAR2A, Clone: Hs-36 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  PRKAR2A (protein kinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The PRKAR2A subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). In sperm, this antigen can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction._x000D_ _x000D_
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by protein-A affinity chromatography.
• Datasheet
• References
  *Peknicova J, Chladek D, Hozak P: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for_x000D_ male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol. 2005 Jan;53(1):42-9. _x000D_
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  PRKAR2A   monoclonal  
• Gene symbol
  PRKAR2A, PRKAR2A-AS1
• Short name
  Anti-PRKAR2A Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgM
• Alternative name
  Mouse Monoclonal to PRKAR2A
• Alternative technique
  antibodies
• Alternative to gene target
  protein kinase, cAMP-dependent, regulatory, type II, alpha, PKR2 and PRKAR2, PRKAR2A and IDBG-33721 and ENSG00000114302 and 5576, protein kinase A catalytic subunit binding, Plasma membranes, Prkar2a and IDBG-200449 and ENSMUSG00000032601 and 19087, PRKAR2A and IDBG-646091 and ENSBTAG00000014205 and 100139910

Gene info

• Identity
  HGNC:9391
• Gene
  PRKAR2A
• Long gene name
  protein kinase cAMP-dependent type II regulatory subunit alpha
• Synonyms gene
  • PRKAR2
• Synonyms gene name
  • protein kinase, cAMP-dependent, regulatory, type II, alpha
  • protein kinase, cAMP-dependent, regulatory subunit type II alpha
• Locus
  3p21.31
• Discovery year
  1988-07-18
• Entrez gene record
  5576
• Pubmed identfication
  • 9676433
• VEGA ID
  OTTHUMG00000133540

Gene info

• Identity
  HGNC:40471
• Gene
  PRKAR2A-AS1
• Long gene name
  PRKAR2A antisense RNA 1
• Synonyms gene name
  • PRKAR2A antisense RNA 1 (non-protein coding)
• Locus
  3p21.31
• Discovery year
  2011-07-28
• Entrez gene record
  100506637
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000156671

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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