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Long name
Mouse Monoclonal Antibody against Human PKAc, Clone: 6D2.1
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Antigen
PKAc
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Species reactivity
Human (Homo sapiens), Other not determined
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
6D2.1
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Applications
WB
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Immunogen
Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha)._x000D_
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Format conjugation
purified; unconjugated
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Specificity
The antibody 6D2.1 strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog. _x000D_
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Research area
Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human PKAc, Clone: 6D2.1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit – PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes._x000D_
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
Contact us for the most recent list of references
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
