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Long name
Mouse Monoclonal Antibody against Human p21Waf1, Clone: WA-1
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Antigen
p21Waf1
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Species reactivity
Human (Homo sapiens), Non-Human (Homo sapiens) Primates
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
WA-1
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Applications
WB, IHC(P), ICC, ELISA
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Immunogen
Human p21 protein
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Format conjugation
purified; unconjugated
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Specificity
The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.
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Research area
Transcription Regulation & Nuclear Proteins (Veterinary), Transcription Regulation & Nuclear Proteins (Human), Cyclin Signaling (Veterinary), Cyclin Signaling (Human), Cyclin Signaling
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human p21Waf1, Clone: WA-1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
The tumour suppressor p21Waf1 (Wild-type p53-Activated Fragment 1; also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Kolar Z, Murray PG, Scott K, Harrison A, Vojtesek B, Dusek J: Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer._x000D_ Mol Pathol. 2000 Feb;53(1):15-8. _x000D_
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
