NG2 / Chondroitin sulfate proteoglycan 4 monoclonal antibody

• Catalog number
  1P-199-T025
• Price
  Please ask
• Size
  25 assays

• Long name
  Mouse Monoclonal Antibody against Human NG2 / Chondroitin sulfate proteoglycan 4, Clone: 7.1
• Antigen
  NG2 / Chondroitin sulfate proteoglycan 4
• Species reactivity
  Human (Homo sapiens)
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  7.1
• Applications
  FC, IP, WB, ICC
• Immunogen
  Human bone marrow stromal cells infected with SV-40
• Format conjugation
  PE
• Specificity
  The mouse monoclonal antibody 7.1 recognizes NG2, the melanoma-associated chondroitin sulfate proteoglycan 4 of Mw approximately 220-300 kDa.
• Research area
  CD and Related Antigens (Human)
• Storage buffer
  The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
• Shipping and storage
  Mouse Monoclonal Antibody against Human NG2 / Chondroitin sulfate proteoglycan 4, Clone: 7.1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  NG2 / Chondroitin sulfate proteoglycan 4 is expressed on glial cell populations, but not on normal hepatopoietic cells. It is an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells, where it plays role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes, and supports signaling pathways important for tumor invasion and growth. NG2 also serves as an AML blast tumor marker associated with poor prognosis.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by affinity chromatography
• Datasheet
• References
  *Cattaruzza S, Nicolosi PA, Braghetta P, Pazzaglia L, Benassi MS, Picci P, Lacrima K, Zanocco D, Rizzo E, Stallcup WB, Colombatti A, Perris R: NG2/CSPG4-collagen type VI interplays putatively involved in the microenvironmental control of tumour engraftment and local expansion. J Mol Cell Biol. 2013 Jun;5(3):176-93., *Girolamo F, Dallatomasina A, Rizzi M, Errede M, Wälchli T, Mucignat MT, Frei K, Roncali L, Perris R, Virgintino D: Diversified expression of NG2/CSPG4 isoforms in glioblastoma and human foetal brain identifies pericyte subsets. PLoS One. 2013 Dec 26;8(12):e84883., Emerenciano M, Renaud G, Sant'Ana M, Barbieri C, Passetti F, Pombo-de-Oliveira MS: Challenges in the use of NG2 antigen as a marker to predict MLL rearrangements in multi-center studies. Leuk Res. 2011 Aug;35(8):1001-7., *Petrovici K, Graf M, Hecht K, Reif S, Pfister K, Schmetzer H: Use of NG2 (7.1) in AML as a tumor marker and its association with a poor prognosis. Cancer Genomics Proteomics. 2010 Jul-Aug;7(4):173-80.
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  NG2   Chondroitin   sulfate   proteoglycan   monoclonal  
• Gene symbol
  NPM1P2, RNF5, CSPG4
• Short name
  Anti-NG2 / Chondroitin sulfate proteoglycan 4 Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG1
• Alternative name
  Mouse Monoclonal to NG2 / Chondroitin sulfate proteoglycan 4
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:7922
• Gene
  NPM1P2
• Long gene name
  nucleophosmin 1 pseudogene 2
• Synonyms gene
  • NPMP2
• Synonyms gene name
  • nucleophosmin 1 (nucleolar phosphoprotein B23, numatrin) pseudogene 2
• Synonyms
  • NG2-6
• GenBank acession
  • X54171
• Locus
  18q11.2
• Discovery year
  1993-08-24
• Entrez gene record
  4871
• Pubmed identfication
  • 8471164
  • 2157848
• VEGA ID
  OTTHUMG00000179428

Gene info

• Identity
  HGNC:10068
• Gene
  RNF5
• Long gene name
  ring finger protein 5
• Synonyms gene name
  • ring finger protein 5, E3 ubiquitin protein ligase
• Synonyms
  • NG2
  • G16
  • RING5
  • RMA1
• GenBank acession
  • U89336
• Locus
  6p21.32
• Discovery year
  1997-10-16
• Entrez gene record
  6048
• Pubmed identfication
  • 9533025
• RefSeq identity
  • NM_006913
• Classification
  • MicroRNA protein coding host genes
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000031093

Gene info

• Identity
  HGNC:2466
• Gene
  CSPG4
• Long gene name
  chondroitin sulfate proteoglycan 4
• Synonyms gene name
  • chondroitin sulfate proteoglycan 4 (melanoma-associated)
• Synonyms
  • MCSPG
  • MEL-CSPG
  • MSK16
  • NG2
  • MCSP
  • HMW-MAA
  • CSPG4A
• Synonyms name
  • melanoma-associated chondroitin sulfate proteoglycan
• GenBank acession
  • X96753
  • AY359468
• Locus
  15q24.2
• Discovery year
  1997-11-28
• Entrez gene record
  1464
• Pubmed identfication
  • 8790396
  • 16407841
• RefSeq identity
  • NM_001897
• Classification
  • Proteoglycans
• VEGA ID
  OTTHUMG00000142836

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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