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Long name
Rabbit Polyclonal Antibody against Human MEK 1/2 / MAPKK 1/2, Clone: PAb
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Antigen
MEK 1/2
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Polyclonal Antibody (PAb)
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Clone
Polyclonal
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Applications
WB
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Immunogen
Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of human MAPK Kinase 1. The immunogen sequence is highly conserved within species (human, mouse, rat, hamster, bovine etc.).
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Format conjugation
purified; unconjugated
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Specificity
The polyclonal antibody reacts with MEK 1/2. MEK 1 and MEK 2 are integral components of the MAP kinase cascade, an important pathway for cell growth and differentiation._x000D_ _x000D_ _x000D_
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Research area
Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine)
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Concentration
1 mg/ml
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Storage buffer
The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storage
Rabbit Polyclonal Antibody against Human MEK 1/2 / MAPKK 1/2, Clone: PAb is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade – an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors._x000D_ _x000D_
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified from rabbit serum by affinity chromatography
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References
Contact us for the most recent list of references
