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Long name
Rabbit Polyclonal Antibody against Human LIME, Clone: PAb
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Antigen
LIME
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
Mouse
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Clonality
Polyclonal Antibody (PAb)
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Clone
Polyclonal
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Applications
WB, IHC(P)
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Immunogen
Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
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Format conjugation
purified; unconjugated
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Specificity
The polyclonal antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
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Research area
Adaptor Proteins (Human), Adaptor Proteins
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Rabbit Polyclonal Antibody against Human LIME, Clone: PAb is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified from rabbit serum by precipitation methods
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References
*Brdickova N, Brdicka T, Angelisova P, Horvath O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsi V. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8_x000D_ coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
