Intra-Acrosomal Protein monoclonal antibody

• Catalog number
  11-281-C100
• Price
  Please ask
• Size
  100ug

• Long name
  Mouse Monoclonal Antibody against Dog Intra-Acrosomal Protein, Clone: Ds-2
• Antigen
  Intra-Acrosomal Protein (Canine)
• Species reactivity
  Canine (Dog)
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  Ds-2
• Applications
  ICC, WB
• Immunogen
  Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
• Format conjugation
  purified; unconjugated
• Specificity
  The antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
• Research area
  Fertility & Reproduction (Veterinary), Fertility & Reproduction
• Concentration
  1 mg/ml
• Storage buffer
  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Shipping and storage
  Mouse Monoclonal Antibody against Dog Intra-Acrosomal Protein, Clone: Ds-2 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by protein-A affinity chromatography
• Datasheet
• References
  *Geussova G, Peknicova J, Capkova J, Kalab P, Moos J, Philimonenko VV, Hozak P: Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction. Andrologia. 1997 Sep-Oct;29(5):261-8. _x000D_
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  Intra-Acrosomal   Protein   monoclonal  
• Short name
  Anti-Intra-Acrosomal Protein Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG
• Alternative name
  Mouse Monoclonal to Intra-Acrosomal Protein (Canine)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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