HLA-Class I monoclonal antibody
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Catalog number11-242-C025
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PricePlease ask
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Size25ug
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Long nameMouse Monoclonal Antibody against Human HLA-Class I, Clone: MEM-147
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AntigenHLA-Class I
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Species reactivityHuman (Homo sapiens)
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Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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ClonalityMonoclonal Antibody (Mab)
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CloneMEM-147
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ApplicationsFC, IP, WB
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ImmunogenPHA-activated peripheral blood lymphocytes
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Format conjugationpurified; unconjugated
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SpecificityThe antibody MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. _x000D_ The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
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Research areaMHC (Major Histocompatibility Complex) and Related Antigens (Human)
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Concentration1 mg/ml
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Storage bufferPhosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storageMouse Monoclonal Antibody against Human HLA-Class I, Clone: MEM-147 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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BackgroundHLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by protein-A affinity chromatography
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References*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84., *Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck_x000D_ and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8., *Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32_x000D_ and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug;53(6):440-6. _x000D_
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PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolHLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DQA2, HLA-DPB1, HLA-DQB2, HLA-W, HLA-DRA, HLA-S
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Short nameAnti-HLA-Class I Mab
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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HostMouse (Mus musculus)
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IsotypeIgG1
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Alternative nameMouse Monoclonal to HLA-Class I
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DP alpha 2 (pseudogene)
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Synonyms gene
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DO alpha
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DQ alpha 1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, N (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-10-23
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Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DQ alpha 2
-
Synonyms gene
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
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Long gene namemajor histocompatibility complex, class II, DP beta 1
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Synonyms gene
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Locus
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Discovery year2001-06-22
-
Entrez gene record
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DQ beta 2
-
Synonyms gene
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, W (pseudogene)
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Synonyms gene
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Synonyms gene name
- HLA-80 pseudogene
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GenBank acession
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Locus
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Discovery year2004-01-16
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Entrez gene record
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Pubmed identfication
-
RefSeq identity
-
Classification
- Histocompatibility complex
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VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
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Long gene namemajor histocompatibility complex, class II, DR alpha
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Synonyms gene
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Locus
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Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class I, S (pseudogene)
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Synonyms
-
GenBank acession
-
Locus
-
Discovery year2002-10-23
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Testing erythrocytes to determine presence or absence of blood-group antigens, testing of serum to determine the presence or absence of antibodies to these antigens, and selecting biocompatible blood by crossmatching samples from the donor against samples from the recipient. Crossmatching is performed prior to transfusion.
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Tree numbers
- E01.370.225.625.120
- E01.370.225.812.385.120
- E05.200.625.120
- E05.200.812.385.120
- E05.478.594.385.120
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data