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Long name
Mouse Monoclonal Antibody against Human Granzyme B, Clone: CLB-GB11
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Antigen
Granzyme B
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Species reactivity
Human (Homo sapiens), Non-Human (Homo sapiens) Primates
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
CLB-GB11
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Applications
FC
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Immunogen
Human NK cell line YT-INDY-derived granzyme B_x000D_
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Format conjugation
purified; unconjugated
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Specificity
The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed in activated Tc cells and NK cells. _x000D_
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Research area
Apoptosis (Veterinary), Apoptosis (Human)
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Concentration
1 mg/ml
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Storage buffer
Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
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Shipping and storage
Mouse Monoclonal Antibody against Human Granzyme B, Clone: CLB-GB11 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death. _x000D_
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Sun Y, Schmitz JE, Acierno PM, Santra S, Subbramanian RA, Barouch DH, Gorgone DA, Lifton MA, Beaudry KR, Manson K, Philippon V, Xu L, Maecker HT, Mascola JR, Panicali D, Nabel GJ, Letvin NL: Dysfunction of simian immunodeficiency virus/simian human immunodeficiency_x000D_ virus-induced IL-2 expression by central memory CD4+ T lymphocytes. J Immunol. 2005 Apr 15;174(8):4753-60._x000D_ , *Rissoan MC, Duhen T, Bridon JM, Bendriss-Vermare N, Péronne C, de Saint Vis B, Brière F, Bates EE: Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells. Blood. 2002 Nov 1;100(9):3295-303._x000D_ , *Tak PP, Spaeny-Dekking L, Kraan MC, Breedveld FC, Froelich CJ, Hack CE: The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA). Clin Exp Immunol. 1999 May;116(2):366-70._x000D_ , *Spaeny-Dekking EH, Hanna WL, Wolbink AM, Wever PC, Kummer JA, Swaak AJ, Middeldorp JM, Huisman HG, Froelich CJ, Hack CE: Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J Immunol. 1998 Apr 1;160(7):3610-6._x000D_
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
