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Long name
Rabbit Polyclonal Antibody against Mouse GABA B receptor GB2 subunit, Clone: PAb
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Antigen
GABA B receptor GB2 subunit
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Species reactivity
Mouse (Mus musculus), Rat, Other species Not tested
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Polyclonal Antibody (PAb)
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Clone
Polyclonal
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Applications
WB, ICC
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Immunogen
Synthetic peptide (coupled with THG) derived from the last 23 aa of mouse GABA B receptor 2. 100% homology with human GB2._x000D_
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Format conjugation
purified; unconjugated
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Specificity
The polyclonal antibody recognizes C-terminus of gamma-aminobutyric acid (GABA) B receptor 2 (recognized epitope: the last 23 aa). GB2 apparent MW ~120 kDa._x000D_
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Research area
Neurobiology (Rodent)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Rabbit Polyclonal Antibody against Mouse GABA B receptor GB2 subunit, Clone: PAb is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
GABA B receptor is a G-protein-coupled inhibitory receptor of gamma-aminobutyric acid (GABA), and has important functions in brain by inhibition of adenylyl cyclase and modulation of G-protein-gated Ca2+ and K+ channels. GABA B receptor is comprised of two subunits, GB1 and GB2 with N-terminal extracellular and C-terminal intracellular domains. The GB1 subunit plays a critical role in ligand binding, whereas the GB2 subunit contains the determinants required for G-protein signaling. Multiple allosteric interactions between the two subunits are required for correct functioning of the receptor. There are two N-terminal splice variants of GB1 subunit, termed GB1a and GB1b; their expression in the central nervous system changes during the ontogenesis and differs between various regions of the brain.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified from rabbit serum by affinity chromatography
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References
Contact us for the most recent list of references
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Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
