-
Long name
Mouse Monoclonal Antibody against Sheep Cytokeratin 8, Clone: C-43
-
Antigen
Cytokeratin 8
-
Species reactivity
Sheep, Human (Homo sapiens), Bovine, Rabbit, Porcine
-
Negative species reactivity
Hamster, Mouse, Xenopus, Rat, Chicken
-
Clonality
Monoclonal Antibody (Mab)
-
Clone
C-43
-
Applications
IP, WB, IHC(P), ICC
-
Immunogen
Cytoskeleton preparation from HeLa human cervix carcinoma cell line.
-
Format conjugation
purified; unconjugated
-
Specificity
The antibody C-43 reacts with Cytokeratin 8 (52.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
-
Research area
Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Human), Cytoskeleton: Intermediate Filaments
-
Concentration
1 mg/ml
-
Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
-
Shipping and storage
Mouse Monoclonal Antibody against Sheep Cytokeratin 8, Clone: C-43 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
-
Background
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
-
Purity
> 95% (by SDS-PAGE)
-
Purification method
Purified by protein-A affinity chromatography
-
-
References
*Kovarik J et al.: J Tumor Marker Oncol. 5, 219 (1990)._x000D_ _x000D_ , *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. _x000D_
-
Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translation
anticorps