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Long name
Mouse Monoclonal Antibody against Human Cdk1 / p34Cdc2, Clone: POH-1
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Antigen
Cdk1
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Species reactivity
Human (Homo sapiens), Non-Human (Homo sapiens) Primates, Bovine
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Negative species reactivity
Drosophila, Mouse, Xenopus, Rat
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Clonality
Monoclonal Antibody (Mab)
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Clone
POH-1
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Applications
IP, WB, IHC(P), ICC
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Immunogen
Bacterially expressed full-length human Cdk1 protein
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Format conjugation
purified; unconjugated
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Specificity
The antibody POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue. _x000D_
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Research area
Cyclin Signaling (Veterinary), Cyclin Signaling (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Veterinary), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Cyclin Signaling, Phosphorylation (Kinases, Phosphatases, Phosphotyrosine)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human Cdk1 / p34Cdc2, Clone: POH-1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Lukas J, Draetta G, Bartek J: Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur J Biochem. 1992 Jul 1;207(1):169-76.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
