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Long name
Mouse Monoclonal Antibody against Human CD68, Clone: Y1/82A
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Antigen
CD68
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
Y1/82A
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Applications
FC, IHC(F)
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Immunogen
Lysosomal contents of lung macrophages
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Format conjugation
purified; unconjugated
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Specificity
The mouse monoclonal antibody Y1/82A recognizes CD68 (LAMP4), a 110 kDa glycoprotein expressed mainly in cytoplasmic granules of monocytes/macrophages, granulocytes, and dendritic cells.
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Research area
CD and Related Antigens (Human)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human CD68, Clone: Y1/82A is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD68 (also known as LAMP4 or SCARD1) is a 110 kDa type I transmembrane glycoprotein of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and the scavenger receptor family. Although CD68 primarily localizes to lysosomes and endosomes, its fraction circulates to the cell surface. By the heavily glycosylated extracellular domain CD68 binds to tissue- and organ-specific lectins or selectins. It is expressed mainly in cytoplasmic granules of monocytes/macrophages, granulocytes, and dendritic cells, but also e.g. in a proportion of epithelial tumours (diagnosis of poorly differentiated neoplasms).
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Holness CL, Simmons DL: Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood. 1993 Mar 15;81(6):1607-13., *Yamagami S, Yokoo S, Amano S, Ebihara N: Characterization of bone marrow derived cells in the substantia propria of the human conjunctiva. Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4476-81., *I A Doussis, K C Gatter, and D Y Mason: CD68 reactivity of non-macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 April; 46(4): 334–336. , *Mack CL, Tucker RM, Sokol RJ, Karrer FM, Kotzin BL, Whitington PF, Miller SD: Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation. Pediatr Res. 2004 Jul;56(1):79-87., *Ulanova M, Tarkowski A, Hahn-Zoric M, Hanson LA: The Common vaccine adjuvant aluminum hydroxide up-regulates accessory properties of human monocytes via an interleukin-4-dependent mechanism. Infect Immun. 2001 Feb;69(2):1151-9., *Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non-macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr;46(4):334-6.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
