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Long name
Mouse Monoclonal Antibody against Human CD45R0, Clone: UCHL1
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Antigen
CD45R0
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
UCHL1
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Applications
FC, IP, IHC(P), IHC(F)
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Immunogen
Human IL-2 dependent T cells
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Format conjugation
PE
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Specificity
The antibody UCHL1 recognizes CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes._x000D_ HLDA III; WS Code NL 826_x000D_ HLDA III; WS Code T 128_x000D_ HLDA IV; WS Code NL 31_x000D_ HLDA V; WS Code BP BP460_x000D_ HLDA V; WS Code T T-081_x000D_ HLDA V; WS Code T T-CD45.43_x000D_ _x000D_
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Research area
Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), CD and Related Antigens (Human)
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Storage buffer
The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storage
Mouse Monoclonal Antibody against Human CD45R0, Clone: UCHL1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD45R0 is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by affinity chromatography
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References
, * Beverley PC.: Human T cell subsets. Immunol Lett. 1987 Apr;14(4):263-7., *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987)._x000D_ _x000D_ , *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)._x000D_
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
