CD45 / LCA monoclonal antibody

• Catalog number
  1P-222-T025
• Price
  Please ask
• Size
  25 assays

• Long name
  Mouse Monoclonal Antibody against Human CD45 / LCA, Clone: MEM-28
• Antigen
  CD45
• Species reactivity
  Human (Homo sapiens)
• Negative species reactivity
  Equine (Horse)
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  MEM-28
• Applications
  FC, IP, WB, IHC(P), ICC
• Immunogen
  Human thymocytes and T lymphocytes.
• Format conjugation
  PE
• Specificity
  The antibody MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. _x000D_ HLDA III; WS Code NL 833a
• Research area
  Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), CD and Related Antigens (Human)
• Storage buffer
  The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
• Shipping and storage
  Mouse Monoclonal Antibody against Human CD45 / LCA, Clone: MEM-28 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by affinity chromatography
• Datasheet
• References
  *Heneberg P, Riegerová K, Kučera P: Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro. PLoS One. 2015 Nov 12;10(11):e0142953. , *Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95., *McCormack E, Mujić M, Osdal T, Bruserud Ø, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555., *Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34. _x000D_ , *Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61., *Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M: Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011 Jul;90(1):5-19., *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84., *Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck_x000D_ and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8., *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD45   LCA   monoclonal  
• Gene symbol
  PTPRC, CLTA, PTPRCAP, GUCY2D
• Short name
  Anti-CD45 / LCA Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG1
• Alternative name
  Mouse Monoclonal to CD45 / LCA
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9666
• Gene
  PTPRC
• Long gene name
  protein tyrosine phosphatase receptor type C
• Synonyms gene
  • CD45
• Synonyms
  • LCA
  • T200
  • GP180
• GenBank acession
  • Y00062
• Locus
  1q31.3-q32.1
• Discovery year
  1986-01-01
• Entrez gene record
  5788
• Pubmed identfication
  • 2169617
• RefSeq identity
  • NM_002838
• Classification
  • Protein tyrosine phosphatases receptor type
  • Fibronectin type III domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000035702
• Locus Specific Databases
  • PTPRCbase: Mutation registry for CD45 deficiency
  • LRG_95

Gene info

• Identity
  HGNC:2090
• Gene
  CLTA
• Long gene name
  clathrin light chain A
• Synonyms gene name
  • clathrin, light polypeptide (Lca)
  • clathrin, light chain A
• Synonyms
  • Lca
• Locus
  9p13.3
• Discovery year
  1991-08-08
• Entrez gene record
  1211
• Pubmed identfication
  • 7713494
• RefSeq identity
  • NM_007096
• Classification
  • Clathrin subunits
• VEGA ID
  OTTHUMG00000019896

Gene info

• Identity
  HGNC:9667
• Gene
  PTPRCAP
• Long gene name
  protein tyrosine phosphatase receptor type C associated protein
• Synonyms
  • LPAP
  • CD45-AP
• Locus
  11q13.2
• Discovery year
  1996-04-22
• Entrez gene record
  5790
• RefSeq identity
  • NM_005608
• VEGA ID
  OTTHUMG00000150325

Gene info

• Identity
  HGNC:4689
• Gene
  GUCY2D
• Long gene name
  guanylate cyclase 2D, retinal
• Synonyms gene
  • CORD6
  • LCA
  • GUC2D
  • GUC1A4
• Synonyms gene name
  • cone rod dystrophy 6
  • guanylate cyclase 2D, membrane (retina-specific)
• Synonyms
  • retGC
  • RETGC-1
  • ROS-GC1
  • CYGD
  • LCA1
• Synonyms name
  • rod outer segment membrane guanylate cyclase
  • retinal guanylate cyclase 1
• GenBank acession
  • L26921
• Locus
  17p13.1
• Discovery year
  1993-11-09
• Entrez gene record
  3000
• Pubmed identfication
  • 1356371
  • 12552567
• RefSeq identity
  • NM_000180
• Classification
  • Guanylate cyclase receptors
• VEGA ID
  OTTHUMG00000108169
• Locus Specific Databases
  • Mutations of the Retinal Guanylate Cyclase Gene and the Retinal Guanylate Cyclase-activating Protein Gene

MeSH Data

• Name
  CD4 Lymphocyte Count
• Concept
  Scope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
• Tree numbers
  • E01.370.225.500.195.107.595.500.150
  • E01.370.225.625.107.595.500.150
  • E05.200.500.195.107.595.500.150
  • E05.200.625.107.595.500.150
  • E05.242.195.107.595.500.150
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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