-
Long name
Mouse Monoclonal Antibody against Human CD38, Clone: HIT2
-
Antigen
CD38
-
Species reactivity
Human (Homo sapiens)
-
Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
-
Clonality
Monoclonal Antibody (Mab)
-
Clone
HIT2
-
Applications
FC, WB, IHC(P), IHC(F)
-
Immunogen
Human thymocytes in foetus
-
Format conjugation
biotin
-
Specificity
The antibody HIT2 reacts with CD38 (T10), a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells.HLDA III; WS Code T 155
-
Research area
CD and Related Antigens (Human)
-
Concentration
1 mg/ml
-
Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
-
Shipping and storage
Mouse Monoclonal Antibody against Human CD38, Clone: HIT2 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
-
Background
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.
-
Purity
> 95% (by SDS-PAGE)
-
Purification method
Purified by affinity chromatography
-
-
References
*Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15(4):e61-71., *Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center_x000D_ population to express AID. Blood. 2007 Mar 15;109(6):2545-52., *Rozková D, Novotná L, Pytlík R, Hochová I, Kozák T, Bartůnková J, Spísek R: Toll-like receptors on B-CLL cells: expression and functional consequences of their stimulation. Int J Cancer. 2010 Mar 1;126(5):1132-43., *Leukocyte Typing III., McMichael AJ et al (Eds.), Oxford University Press (1987).
-
Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translation
anticorps
