CD30 / Ki-1 monoclonal antibody
#
-
Catalog number1F-455-T025
-
Price:
-
Size25 assays
-
-
Long nameMouse Monoclonal Antibody against Human CD30 / Ki-1, Clone: MEM-268
-
AntigenCD30
-
Species reactivityHuman (Homo sapiens)
-
Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
-
ClonalityMonoclonal Antibody (Mab)
-
CloneMEM-268
-
ApplicationsFC, IHC(P)
-
ImmunogenExpression vector containing CD30 cDNA (booster suspension of THP-1 cell line)
-
Format conjugationFITC
-
SpecificityThe antibody MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).
-
Research areaCD and Related Antigens (Human)
-
Storage bufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
-
Shipping and storageMouse Monoclonal Antibody against Human CD30 / Ki-1, Clone: MEM-268 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
-
BackgroundCD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.
-
Purity> 95% (by SDS-PAGE)
-
Purification methodPurified by affinity chromatography
-
References*Pavlov I, Martins TB, Delgado JC: Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing. Clin Vaccine Immunol. 2009 Sep;16(9):1327-31.
-
PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translationanticorps
-
Gene target
-
Gene symbolTNFRSF8
-
Short nameAnti-CD30 / Ki-1 Mab
-
TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
-
HostMouse (Mus musculus)
-
IsotypeIgG
-
Alternative nameMouse Monoclonal to CD30 / Ki-1
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameTNF receptor superfamily member 8
-
Synonyms gene
-
Synonyms gene name
- tumor necrosis factor receptor superfamily, member 8
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1992-01-10
-
Entrez gene record
-
Pubmed identfication
-
Classification
- CD molecules
- Tumor necrosis factor receptor superfamily
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data