CD20 monoclonal antibody

• Catalog number
  1F-638-T100
• Price
  Please ask
• Size
  100 assays

• Long name
  Mouse Monoclonal Antibody against Human CD20, Clone: 2H7
• Antigen
  CD20
• Species reactivity
  Human (Homo sapiens), Non-Human (Homo sapiens) Primates
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  2H7
• Applications
  FC, IP, IHC(F)
• Immunogen
  Human tonsillar B cells
• Format conjugation
  FITC
• Specificity
  The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. _x000D_ HLDA IV; WS Code B201
• Research area
  CD Antigens & MHC (Veterinary), Calcium Signaling (Human), CD and Related Antigens (Human)
• Storage buffer
  The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
• Shipping and storage
  Mouse Monoclonal Antibody against Human CD20, Clone: 2H7 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by affinity chromatography
• Datasheet
• References
  , *Polyak MJ, Ayer LM, Szczepek AJ, Deans JP: A cholesterol-dependent CD20 epitope detected by the FMC7 antibody. Leukemia. 2003 Jul;17(7):1384-9., *Hayden-Ledbetter MS, Cerveny CG, Espling E, Brady WA, Grosmaire LS, Tan P, Bader R, Slater S, Nilsson CA, Barone DS, Simon A, Bradley C, Thompson PA, Wahl AF, Ledbetter JA: CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells. Clin Cancer Res. 2009 Apr 15;15(8):2739-46., *Diehl SA, Schmidlin H, Nagasawa M, van Haren SD, Kwakkenbos MJ, Yasuda E, Beaumont T, Scheeren FA, Spits H: STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation. J Immunol. 2008 Apr 1;180(7):4805-15., *Deans JP, Robbins SM, Polyak MJ, Savage JA: Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment. J Biol Chem. 1998 Jan 2;273(1):344-8., *Deans JP, Kalt L, Ledbetter JA, Schieven GL, Bolen JB, Johnson P: Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J Biol Chem. 1995 Sep 22;270(38):22632-8., *Rose AL, Smith BE, Maloney DG: Glucocorticoids and rituximab in vitro: synergistic direct antiproliferative and _x000D_ apoptotic effects. Blood. 2002 Sep 1;100(5):1765-73., *Polyak MJ, Deans JP: Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. 2002 May 1;99(9):3256-62., *Chan HT, Hughes D, French RR, Tutt AL, Walshe CA, Teeling JL, Glennie MJ, Cragg MS: CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts. Cancer Res. 2003 Sep 1;63(17):5480-9., *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., *Leukocyte Typing III., McMichael M.J. et al. (Eds.), Oxford University Press (1987); p.611., *Leukocyte Typing Human B Cell and B cell blast-associated Surface Molecules defined with Monoclonal Antibodies, Clerk et al., 1984, pg. 339-346., *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD20   monoclonal  
• Gene symbol
  MS4A1
• Short name
  Anti-CD20 Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG2b
• Alternative name
  Mouse Monoclonal to CD20
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:7315
• Gene
  MS4A1
• Long gene name
  membrane spanning 4-domains A1
• Synonyms gene
  • CD20
• Synonyms gene name
  • membrane-spanning 4-domains, subfamily A, member 1
• Synonyms
  • B1
  • Bp35
  • FMC7
• GenBank acession
  • M27394
• Locus
  11q12.2
• Discovery year
  1989-05-23
• Entrez gene record
  931
• Pubmed identfication
  • 2448768
• Classification
  • Membrane spanning 4-domains
  • CD molecules
• VEGA ID
  OTTHUMG00000167614
• Locus Specific Databases
  • LRG_140

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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