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Long name
Mouse Monoclonal Antibody against Human CD195 / CCR5, Clone: T21/8
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Antigen
CD195 / CCR5
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
T21/8
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Applications
FC, IP, WB, ICC, ELISA
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Immunogen
CCR5 peptide (Met1-Lys22) KLH conjugate
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Format conjugation
purified; unconjugated
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Specificity
The mouse monoclonal antibody T21/8 recognizes the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.
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Research area
Cytokine Signaling (Human), CD and Related Antigens (Human)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human CD195 / CCR5, Clone: T21/8 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Mascalchi P, Lamort AS, Salomé L, Dumas F: Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes. Biochem Biophys Res Commun. 2012 Jan 6;417(1):409-13, *Monde K, Maeda Y, Tanaka Y, Harada S, Yusa K: Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5. J Biol Chem. 2007 Dec 21;282(51):36923-32., *Hüttenrauch F, Pollok-Kopp B, Oppermann M: G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers. J Biol Chem. 2005 Nov 11;280(45):37503-15., *Pollok-Kopp B, Schwarze K, Baradari VK, Oppermann M: Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
