CD180 monoclonal antibody
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Catalog number1P-637-T100
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Price:
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Size100 assays
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Long nameMouse Monoclonal Antibody against Human CD180, Clone: G28-8
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AntigenCD180
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Species reactivityHuman (Homo sapiens)
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Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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ClonalityMonoclonal Antibody (Mab)
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CloneG28-8
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ApplicationsFC, IP, FUNC
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ImmunogenHuman tonsillar B cells
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Format conjugationPE
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SpecificityThe mouse monoclonal antibody G28-8 recognizes CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
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Research areaCD and Related Antigens (Human)
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Storage bufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storageMouse Monoclonal Antibody against Human CD180, Clone: G28-8 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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BackgroundCD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1 , and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by affinity chromatography
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References*Olson NE, Graves JD, Shu GL, Ryan EJ, Clark EA: Caspase activity is required for stimulated B lymphocytes to enter the cell cycle. J Immunol. 2003 Jun 15;170(12):6065-72., *Clark EA, Shu GL, Lüscher B, Draves KE, Banchereau J, Ledbetter JA, Valentine MA: Activation of human B cells. Comparison of the signal transduced by IL-4 to four _x000D_ different competence signals. J Immunol. 1989 Dec 15;143(12):3873-80., *Valentine MA, Clark EA, Shu GL, Norris NA, Ledbetter JA: Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation. J Immunol. 1988 Jun 15;140(12):4071-8.
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PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolCD180
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Short nameAnti-CD180 Mab
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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HostMouse (Mus musculus)
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IsotypeIgG1
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Alternative nameMouse Monoclonal to CD180
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Alternative techniqueantibodies
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Alternative to gene targetCD180 molecule, LY64 and Ly78 and RP105, CD180 and IDBG-24968 and ENSG00000134061 and 4064, protein binding, Plasma membranes, Cd180 and IDBG-179053 and ENSMUSG00000021624 and 17079, BT.22214 and IDBG-628694 and ENSBTAG00000006360 and 539518
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Gene info
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Identity
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Gene
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Long gene nameCD180 molecule
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Synonyms gene
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Synonyms gene name
- lymphocyte antigen 64 (mouse) homolog, radioprotective, 105kD
- CD180 antigen
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-05-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data