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Long name
Mouse Monoclonal Antibody against Rat CD161, Clone: 10/78
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Antigen
CD161 (rat)
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Species reactivity
Rat
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
10/78
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Applications
FC, IP, WB, IHC(F), RIA
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Immunogen
Splenic cells purified from the LEW rat
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Format conjugation
purified; unconjugated
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Specificity
The mouse monoclonal antibody 10/78 recognizes CD161, an approximately 30 kDa type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. A common epitope on rat CD161a and b isoforms is detected.
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Research area
CD and Related Antigens & MHC (Rodent)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Rat CD161, Clone: 10/78 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major_x000D_ histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26(11):2582-6., *Sedgwick JD, Ford AL, Foulcher E, Airriess R: Central nervous system microglial cell activation and proliferation follows_x000D_ direct interaction with tissue-infiltrating T cell blasts. J Immunol. 1998 Jun 1;160(11):5320-30., *Tliba O, Chauvin A, Le Vern Y, Boulard C, Sbille P: Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats. Vet Res. 2002 May-Jun;33(3):327-32., *Teshima R, Nakamura R, Nakamura R, Hachisuka A, Sawada JI, Shibutani M: Effects of exposure to decabromodiphenyl ether on the development of the immune system in rats. J Health Sci 2008;54(4):382-389, *Chang CJ, Tai KF, Roffler S, Hwang LH: The immunization site of cytokine-secreting tumor cell vaccines influences the_x000D_ trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors. J Immunol. 2004 Nov 15;173(10):6025-32., *Stephens LA, Barclay AN, Mason D: Phenotypic characterization of regulatory CD4+CD25+ T cells in rats. Int Immunol. 2004 Feb;16(2):365-75., *May E, Dorris ML, Satumtira N, Iqbal I, Rehman MI, Lightfoot E, Taurog JD: CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats. J Immunol. 2003 Jan 15;170(2):1099-105.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
