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Long name
Mouse Monoclonal Antibody against Human CD142, Clone: HTF-1
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Antigen
CD142
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
HTF-1
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Applications
FC, WB, ICC, FUNC
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Immunogen
Human brain tissue factor (CD142)
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Format conjugation
purified; unconjugated
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Specificity
The mouse monoclonal antibody HTF-1, also known as HTF1-7B8, recognizes CD142 (tissue factor, coagulation factor III), a type I glycoprotein expressed on endothelial cells, monocytes, macrophages, and platelets upon induction by inflammatory mediators, and expressed constitutively by some tumors, the vasculature, placenta, kidney, and central nervous system._x000D_ HLDA VI; WS Code E016
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Research area
CD and Related Antigens (Human)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human CD142, Clone: HTF-1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD142, also known as coagulation factor III, tissue thromboplastin, and tissue factor. It is a transmembrane glycoprotein, which enables cells to initiate the blood coagulation cascades, and functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. It is the only one factor in the coagulation pathway for which a congenital deficiency has not been described.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Patil R, Ghosh K, Satoskar P, Shetty S: Elevated procoagulant endothelial and tissue factor expressing microparticles in women with recurrent pregnancy loss. PLoS One. 2013 Nov 20;8(11):e81407., *Aass HC1, Øvstebø R, Trøseid AM, Kierulf P, Berg JP, Henriksson CE: Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis. Cytometry A. 2011 Dec;79(12):990-9. doi: 10.1002/cyto.a.21147. _x000D_ , Bucciarelli P, Martinelli I, Artoni A, Passamonti SM, Previtali E, Merati G, Tripodi A, Mannucci PM: Circulating microparticles and risk of venous thromboembolism. Thromb Res. 2012 May;129(5):591-7, *Carson SD, Ross SE, Bach R, Guha A: An inhibitory monoclonal antibody against human tissue factor. Blood. 1987 Aug;70(2):490-3.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
