CD135 monoclonal antibody
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Catalog number1P-587-T025
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PricePlease ask
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Size25 assays
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Long nameMouse Monoclonal Antibody against Human CD135, Clone: BV10A4
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AntigenCD135
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Species reactivityHuman (Homo sapiens)
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Negative species reactivityMouse
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ClonalityMonoclonal Antibody (Mab)
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CloneBV10A4
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ApplicationsFC, IP
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ImmunogenBV-173 leukemic cell line_x000D_
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Format conjugationPE
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SpecificityThe mouse monoclonal antibody BV10A4 (BV10) reacts with CD135 (FLT3, FLK2, STK-1), a 130-160 kDa type I transmembrane receptor tyrosine kinase that is involved in early steps of hematopoiesis. _x000D_
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Research areaPhosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), CD and Related Antigens (Human)
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Storage bufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storageMouse Monoclonal Antibody against Human CD135, Clone: BV10A4 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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BackgroundCD135 / FLT3, also known as FLK2 or STK-1 is a receptor tyrosine kinase that plays important roles in hematopoiesis. After binding of Flt3 ligand (FL), CD135 homodimerizes and stimulates proliferation, differentiation and protects the cell from apoptosis. The loss of CD90 and gain of CD135 expression marks the loss of self-renewal in hematopoietic stem cell population. Detectable CD135 expression appears first at low levels on the surface of primitive multilineage progenitor cells and disappears during defined stages of B-cell development, but is upregulated and maintained during maturation of monocytes. CD135 is also expressed on thymocytes, dendritic cell progenitors and on mature dendritic cells, as well as on various malignant hematopoietic cells._x000D_
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by affinity chromatography
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References*Haylock DN, Horsfall MJ, Dowse TL, Ramshaw HS, Niutta S, Protopsaltis S, Peng L, Burrell C, Rappold I, Buhring HJ, Simmons PJ: Increased recruitment of hematopoietic progenitor cells underlies the ex vivo expansion potential of FLT3 ligand. Blood. 1997 Sep 15;90(6):2260-72._x000D_ , *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)._x000D_
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PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolFLT3
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Short nameAnti-CD135 Mab
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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HostMouse (Mus musculus)
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IsotypeIgG1
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Alternative nameMouse Monoclonal to CD135
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namefms related receptor tyrosine kinase 3
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Synonyms gene name
- fms-related tyrosine kinase 3
- fms related tyrosine kinase 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-07-30
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin like domain containing
- Receptor tyrosine kinases
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data