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Long name
Mouse Monoclonal Antibody against Human CD11b, Clone: ICRF44
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Antigen
CD11b
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Species reactivity
Human (Homo sapiens), Non-Human (Homo sapiens) Primates
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
ICRF44
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Applications
FC, IP, IHC(F), ICC, FUNC
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Immunogen
Rheumatoid synovial cells and human monocytes.
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Format conjugation
PE
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Specificity
The mouse monoclonal antibody ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. _x000D_ HLDA IV, WS Code M047
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Research area
CD and Related Antigens (Human)
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Storage buffer
The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storage
Mouse Monoclonal Antibody against Human CD11b, Clone: ICRF44 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages._x000D_ CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. _x000D_
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by affinity chromatography
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References
*Hua J, Sakamoto K, Nagaoka I: Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the_x000D_ functions of neutrophils. J Leukoc Biol. 2002 Apr;71(4):632-40., *Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int Immunol. 2001 Dec;13(12):1571-81., *Masten BJ, Olson GK, Tarleton CA, Rund C, Schuyler M, Mehran R, Archibeque T, Lipscomb MF: Characterization of myeloid and plasmacytoid dendritic cells in human lung. J Immunol. 2006 Dec 1;177(11):7784-93., *Tieu BC, Lee C, Sun H, Lejeune W, Recinos A 3rd, Ju X, Spratt H, Guo DC, Milewicz D, Tilton RG, Brasier AR: An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice. J Clin Invest. 2009 Dec;119(12):3637-51., *Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23_x000D_ induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta_x000D_ production in primary human monocytes through a pathway dependent on nuclear_x000D_ factor-kappaB. Blood. 2001 May 15;97(10):2932-40.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
