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Long name
Mouse Monoclonal Antibody against Human beta2-Microglobulin, Clone: B2M-02
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Antigen
beta2-Microglobulin
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Species reactivity
Human (Homo sapiens), Porcine
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
B2M-02
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Applications
FC, WB, IHC(P), ELISA
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Immunogen
Purified isolated human beta2-microglobulin.
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Format conjugation
purified; unconjugated
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Specificity
The antibody B2M-02 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes._x000D_
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Research area
CD Antigens & MHC (Veterinary), Serum & Plasma Proteins (Veterinary), Serum & Plasma Proteins (Human), MHC (Major Histocompatibility Complex) and Related Antigens (Human)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human beta2-Microglobulin, Clone: B2M-02 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of_x000D_ human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76., *Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6. _x000D_
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
