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Long name
Mouse Monoclonal Antibody against Human Bcl2, Clone: Bcl-2/100
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Antigen
Bcl2
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
Mouse
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Clonality
Monoclonal Antibody (Mab)
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Clone
Bcl-2/100
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Applications
FC, IP, WB, IHC(P), IHC(F), ICC
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Immunogen
Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
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Format conjugation
purified; unconjugated
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Specificity
The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.
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Research area
Apoptosis (Human)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human Bcl2, Clone: Bcl-2/100 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
Bcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Gugasyan R, Christou A, O'Reilly LA, Strasser A, Gerondakis S: Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA. Cell Death Differ. 2006 Jul;13(7):1235-7., *Soilu-Hänninen M, Ekert P, Bucci T, Syroid D, Bartlett PF, Kilpatrick TJ: Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway. J Neurosci. 1999 Jun 15;19(12):4828-38., *Laflamme C, Israël-Assayag E, Cormier Y: Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis. Eur Respir J. 2003 Feb;21(2):225-31., *Laforge M, Petit F, Estaquier J, Senik A: Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human _x000D_ immunodeficiency virus type 1 is initiated by lysosomal membrane_x000D_ permeabilization, itself induced by the isolated expression of the viral protein _x000D_ Nef. J Virol. 2007 Oct;81(20):11426-40., *And other., *Joubert A, Marais S, Maritz C: Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential_x000D_ interference contrasting technique and Bcl-2 and Bax protein expression levels. Biocell. 2009 Apr;33(1):67-70.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
