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Long name
Mouse Monoclonal Antibody against Human Basic Cytokeratins, Clone: AE3
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Antigen
Basic Cytokeratins
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Species reactivity
Human (Homo sapiens), Non-Human (Homo sapiens) Primates, Mouse (Mus musculus), Rat, Bovine, Rabbit, Chicken
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
AE3
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Applications
WB, IHC(P), IHC(F)
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Immunogen
Human epidermal keratins
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Format conjugation
purified; unconjugated
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Specificity
Mouse monoclonal antibody AE3 recognizes all basic type cytokeratins, namely K1-K8 (58-67 kDa). This antibody reacts with all layers of epidermis (basal layer and above) and is an excellent marker for distinguishing carcinomas from non-epithelial tumours.
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Research area
Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Rodent), Cytoskeleton: Intermediate Filaments (Human)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human Basic Cytokeratins, Clone: AE3 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Hunt RC, Davis AA: Altered expression of keratin and vimentin in human retinal pigment epithelial cells in vivo and in vitro. J Cell Physiol. 1990 Nov;145(2):187-99., *Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8., *Zhang B, Chen Y, Han Z, Ris H, Zhai Z: The role of keratin filaments during nuclear envelope reassembly in Xenopus egg extracts. FEBS Lett. 1998 May 22;428(1-2):52-6., *Yeh IT, Miller AR: Staining of sentinel node reticulum cells with cytokeratin antibody AE3. Arch Pathol Lab Med. 2002 Mar;126(3):248-9., *Listrom MB, Dalton LW: Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2. Am J Clin Pathol. 1987 Sep;88(3):297-301.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
