ARHGEF4 / ASEF1 monoclonal antibody
-
Catalog number
11-723-C100
-
Price
Please ask
-
Size
100ug
-
-
Long name
Mouse Monoclonal Antibody against Human ARHGEF4 / ASEF1, Clone: ARHGEF-08
-
Antigen
ARHGEF4
-
Species reactivity
Human (Homo sapiens)
-
Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
-
Clonality
Monoclonal Antibody (Mab)
-
Clone
ARHGEF-08
-
Applications
FC, WB
-
Immunogen
Recombinant fragment of human ARHGEF4 (amino acids 143-271)
-
Format conjugation
purified; unconjugated
-
Specificity
The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42.
-
Research area
Signaling of Small GTPases (Human)
-
Concentration
1 mg/ml
-
Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
-
Shipping and storage
Mouse Monoclonal Antibody against Human ARHGEF4 / ASEF1, Clone: ARHGEF-08 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
-
Background
ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli – stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
-
Purity
> 95% (by SDS-PAGE)
-
Purification method
Purified by protein-A affinity chromatography
-
-
References
Contact us for the most recent list of references
-
Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translation
anticorps
-
Gene target
-
Gene symbol
ARHGEF4, ARHGEF4-AS1
-
Short name
Anti-ARHGEF4 / ASEF1 Mab
-
Technique
Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
-
Host
Mouse (Mus musculus)
-
Isotype
IgG1
-
Alternative name
Mouse Monoclonal to ARHGEF4 / ASEF1
-
Alternative technique
antibodies
-
Alternative to gene target
Rho guanine nucleotide exchange factor (GEF) 4, ARHGEF4 and IDBG-69993 and ENSG00000136002 and 101930241,50649, Rac guanyl-nucleotide exchange factor activity, Cytoplasm, Arhgef4 and IDBG-143547 and ENSMUSG00000037509 and 226970, ARHGEF4 and IDBG-638970 and ENSBTAG00000015834 and 505852
-
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
ARHGEF4 antisense RNA 1
-
Locus
-
Discovery year
2021-07-12
-
Classification
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
