Sheep anti Rat IgG Fab, conjugated with Horseradish peroxidase, Secondary Antibodies
-
Catalog number
ShARa-Fab-PO
-
Price
Please ask
-
Size
1ml
-
-
Host Source
Sheep
-
Clone Name
Polyclonal
-
Product Category
Veterinary
-
Species Reactivity
Rat
-
Application
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
-
Immunogen
Purified normal Fab of polyclonal IgG isolated from pooled rat serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
-
Purification Method
NA
-
Shipping Conditions
NA
-
Storage conditions
Sheep anti Rat IgG Fab, conjugated with Horseradish peroxidase, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years. Reconstituted at +4ÁC--7 days.
-
Datasheet link
NA
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
-
Latin name
Rattus norvegicus
-
Gene target
-
Short name
anti IgG Fab, conjugated with Horseradish , Secondary Antibodies
-
Technique
anti, Fab, IgG, antibody to, antibody Conjugates, fabs, IgGs
-
Host
Sheep
-
Isotype
NA, Fab fragment, IgG
-
Label
horseradish
-
Species
Rat, Rats, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
-
Alternative name
ovine antibody to Rat Immunoglobulin G Fab, coupled including Horseradish peroxidase, secondary detector Antibody Antibodies
-
Alternative technique
antibodies, seconairy, immunoglobulins
-
MeSH Data
-
Name
-
Concept
Scope note:
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
-
Tree numbers
- E01.370.225.500.620.670.325
- E01.370.225.750.600.670.325
- E05.200.500.620.670.325
- E05.200.750.600.670.325
- E05.393.661.475
-
Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
Product images
Similar products