Sheep anti Rat alpha foetoprotein, conjugated with FITC, Primary Antibodies
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Catalog number
ShARa-AFP-FITC
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Price
Please ask
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Size
1ml
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Host Source
Sheep
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Clone Name
Polyclonal
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Product Category
Veterinary
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Species Reactivity
Rat
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Application
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
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Immunogen
Purified rat alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum using a proprietary multiple step method. Several foetoproteins have been demonstrated in rat foetal serum and amniotic fluid. One of the best studied is the alpha-foetoprotein. It can be easily identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and the alpha-1 globulin fractions. Its molecular weight is 65,000, the carbohydrate content is relatively low. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
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Purification Method
NA
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Shipping Conditions
NA
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Storage conditions
Sheep anti Rat alpha foetoprotein, conjugated with FITC, Primary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years. Reconstituted at +4ÁC--7 days.
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Datasheet link
NA
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Description
The anti alpha foetoprotein, conjugated with FITC, Primary Antibodies is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Short name
anti alpha foetoprotein, conjugated with FITC, Primary Antibodies
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Technique
anti, FITC, antibody to, antibody Conjugates, Fluorescein
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Host
Sheep
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Isotype
NA
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Label
FITC
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Species
Rat, Rats, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
ovine antibody to Rat a foetoprotein, coupled including fluorecein, captor Antibodies
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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