Rabbit anti Pseudomonas testosteroni cells b-Hydroxysteroid dehydrogenase, conjugated with Biotin, Primary Antibodies
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Catalog number
NE079-Bio
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Price
Please ask
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Size
1ml
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Host Source
Rabbit
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Clone Name
Polyclonal
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Product Category
Enzymes, Microbiology
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Species Reactivity
Bacteria
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Application
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
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Immunogen
beta-Hydroxybutyrate dehydrogenase isolated and purified from Pseudomonas testosteroni cells. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
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Purification Method
NA
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Shipping Conditions
NA
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Storage conditions
Rabbit anti Pseudomonas testosteroni cells b-Hydroxysteroid dehydrogenase, conjugated with Biotin, Primary Antibodies philised conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days.
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Datasheet link
NA
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Pseudomonas is a Gram-negative bacteria. Pseudomonas genomic sequences are used in medical research. A problem in hostpital is often the Pseudomonas Aeruginosa containing multi drug resistancy properties that are transmitted through plasmids. The MDR property is transmitter by the R plasmid. Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Rabbit anti Pseudomonas testosteroni cells b-Hydroxysteroid dehydrogenase, conjugated with , Primary Antibodies
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Technique
Rabbit, anti, antibody to, antibody Conjugates
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Host
Rabbit, Rabbits
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Isotype
NA
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Label
Biotin
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Species
Pseudomonas, Pseudomonas
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Alternative name
production species: rabbit antibody to Pseudomonas testosteroni cells b-Hydroxysteroid dehydrogenase, coupled including biotinilated, captor Antibodies
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Alternative technique
rabbit-anti, antibodies
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Tissue
cells
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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