Rabbit anti Cucurbita species Ascorbate oxidase, Primary Antibodies
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Catalog number
NE020-7S
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Price
Please ask
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Size
10mg
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Host Source
Rabbit
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Clone Name
Polyclonal
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Product Category
Enzymes, Microbiology
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Species Reactivity
Plant
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Application
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
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Immunogen
Ascorbate oxidase isolated and purified from cucurbita species. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
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Purification Method
NA
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Shipping Conditions
NA
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Storage conditions
Rabbit anti Cucurbita species Ascorbate oxidase, Primary Antibodies philised IgG fraction is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days.
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Datasheet link
NA
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Monoclonals are usually produced in mouse as host species and polyclonals in rabbit or goat. Multi species reactivity is achieved by selecting consensus epitopes when blasting the gene for a reactive, innugenic epitope that is the same in the different target species.
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About
Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Rabbit anti Cucurbita Ascorbate oxidase, Primary Antibodies
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Technique
Rabbit, anti, antibody to
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Host
Rabbit, Rabbits
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Isotype
NA
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Alternative name
production species: rabbit antibody to Cucurbita species Ascorbate oxidase, captor Antibodies
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Alternative technique
rabbit-anti, antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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